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chicken hs4 insulator  (Addgene inc)


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    Structured Review

    Addgene inc chicken hs4 insulator
    Chicken Hs4 Insulator, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chicken+hs4+insulator/pBT268_(pCA-ATG-intron-tTA2-iiTRE-tdT3Mycii)+(Plasmid+%2336880)/pm32835482-293-158-165
    Average 92 stars, based on 2 article reviews
    chicken hs4 insulator - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Amplification:

    Article Title: Multicopy Targeted Integration for Accelerated Development of High-Producing Chinese Hamster Ovary Cells.
    Article Snippet: .. Plasmids and sequences used for the amplification of genetic elements have been described previously: site A and site T9 homology Page 26 of 41 ACS Paragon Plus Environment ACS Synthetic Biology 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 27 arms, mCherry, NeoR, EF1α, SV40 promoters and poly(A) tails, ZsGreen1-DR expression cassette, Cricetulus griseus codon-optimized EPO were amplified from plasmids described in Pristovšek et al.26; ETN sequence was described in Pristovšek et al.24 Sequence of 100RPU.2 promoter (aka 100 RPU construct 2) was described in Brown et al.27 and was synthesized as gBlock (IDT). tagBFP was amplified from plasmid Addgene#60903, WPRE was amplified from plasmid Addgene#17492, chicken HS4 insulator was amplified from plasmid Addgene#36880. .. Plasmid backbone was cloned from pJ204 plasmid (DNA 2.0).

    Expressing:

    Article Title: Multicopy Targeted Integration for Accelerated Development of High-Producing Chinese Hamster Ovary Cells.
    Article Snippet: .. Plasmids and sequences used for the amplification of genetic elements have been described previously: site A and site T9 homology Page 26 of 41 ACS Paragon Plus Environment ACS Synthetic Biology 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 27 arms, mCherry, NeoR, EF1α, SV40 promoters and poly(A) tails, ZsGreen1-DR expression cassette, Cricetulus griseus codon-optimized EPO were amplified from plasmids described in Pristovšek et al.26; ETN sequence was described in Pristovšek et al.24 Sequence of 100RPU.2 promoter (aka 100 RPU construct 2) was described in Brown et al.27 and was synthesized as gBlock (IDT). tagBFP was amplified from plasmid Addgene#60903, WPRE was amplified from plasmid Addgene#17492, chicken HS4 insulator was amplified from plasmid Addgene#36880. .. Plasmid backbone was cloned from pJ204 plasmid (DNA 2.0).

    Sequencing:

    Article Title: Multicopy Targeted Integration for Accelerated Development of High-Producing Chinese Hamster Ovary Cells.
    Article Snippet: .. Plasmids and sequences used for the amplification of genetic elements have been described previously: site A and site T9 homology Page 26 of 41 ACS Paragon Plus Environment ACS Synthetic Biology 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 27 arms, mCherry, NeoR, EF1α, SV40 promoters and poly(A) tails, ZsGreen1-DR expression cassette, Cricetulus griseus codon-optimized EPO were amplified from plasmids described in Pristovšek et al.26; ETN sequence was described in Pristovšek et al.24 Sequence of 100RPU.2 promoter (aka 100 RPU construct 2) was described in Brown et al.27 and was synthesized as gBlock (IDT). tagBFP was amplified from plasmid Addgene#60903, WPRE was amplified from plasmid Addgene#17492, chicken HS4 insulator was amplified from plasmid Addgene#36880. .. Plasmid backbone was cloned from pJ204 plasmid (DNA 2.0).

    Construct:

    Article Title: Multicopy Targeted Integration for Accelerated Development of High-Producing Chinese Hamster Ovary Cells.
    Article Snippet: .. Plasmids and sequences used for the amplification of genetic elements have been described previously: site A and site T9 homology Page 26 of 41 ACS Paragon Plus Environment ACS Synthetic Biology 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 27 arms, mCherry, NeoR, EF1α, SV40 promoters and poly(A) tails, ZsGreen1-DR expression cassette, Cricetulus griseus codon-optimized EPO were amplified from plasmids described in Pristovšek et al.26; ETN sequence was described in Pristovšek et al.24 Sequence of 100RPU.2 promoter (aka 100 RPU construct 2) was described in Brown et al.27 and was synthesized as gBlock (IDT). tagBFP was amplified from plasmid Addgene#60903, WPRE was amplified from plasmid Addgene#17492, chicken HS4 insulator was amplified from plasmid Addgene#36880. .. Plasmid backbone was cloned from pJ204 plasmid (DNA 2.0).

    Synthesized:

    Article Title: Multicopy Targeted Integration for Accelerated Development of High-Producing Chinese Hamster Ovary Cells.
    Article Snippet: .. Plasmids and sequences used for the amplification of genetic elements have been described previously: site A and site T9 homology Page 26 of 41 ACS Paragon Plus Environment ACS Synthetic Biology 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 27 arms, mCherry, NeoR, EF1α, SV40 promoters and poly(A) tails, ZsGreen1-DR expression cassette, Cricetulus griseus codon-optimized EPO were amplified from plasmids described in Pristovšek et al.26; ETN sequence was described in Pristovšek et al.24 Sequence of 100RPU.2 promoter (aka 100 RPU construct 2) was described in Brown et al.27 and was synthesized as gBlock (IDT). tagBFP was amplified from plasmid Addgene#60903, WPRE was amplified from plasmid Addgene#17492, chicken HS4 insulator was amplified from plasmid Addgene#36880. .. Plasmid backbone was cloned from pJ204 plasmid (DNA 2.0).



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